Comparison of bone absorption capacity of rat osteoclasts cultured by two methods

doi:10.3969/j.issn.2095-4344.2013.20.001

Abstract

Abstract:

BACKGROUND: There are less primary cultured osteoclasts, but a large number of osteoclast-like cells produced by induced culture, which is able to meet the requirements of some experimental studies of bone metabolism. But there are no detailed data to identify whether the osteoclasts and osteoclast-like cells have the similar effect on specific enzymes and bone absorption capacity.
OBJECTIVE: To compare the difference of bone absorption ability between the primary cultured osteoclasts and induce cultured osteoclast-like cells from the rats.
METHODS: The long bones were obtained from the limbs of 24 hours newborn Wistar rates, and the enzyme digestion was used to isolate and culture osteoclasts; then bone marrow mononuclear cells separated from the osteoclasts during culture were added with 1,25(OH)₂D₃ to induce to generate the osteoclast-like cells. The cells were identified with heamtoxylin-eosin staining and tartrate-resistant acid phosphatase staining. The bone absorption ability of the osteoclasts in two groups was compared with toluidine blue staining.
RESULTS AND CONCLUSION: The number of the osteoclast-like cells was 11 times that of osteoclasts at 9 days after induction. The morphology of osteoclast-like cells was similar with that of the primary cultured osteoclasts. The osteoclast-like cells were positive for tartrate-resistant acid phosphatase. Toluidine blue staining results showed there was no significant difference in bone lacuna area and depth produced by the osteoclasts cultured on the bone slices between two groups. These findings show that there are no significant differences in morphology, specific enzymes and bone absorption capacity between osteoclast-like cells and osteoclasts.

Key words: tissue construction, bone tissue construction, osteoclasts, culture, induction, identification, bone absorption, 1,25(OH)₂D₃, osteoclast-like cells, mononuclear cells, Wistar rats

CLC Number:

R318

Wang Zheng-dong,Yan Nan, Pan Feng, Yang Fang-li, Liu Zhi-li, Jiang Hai-bo, Zang Jin, Mu Jun, Bai Shu-ling. Comparison of bone absorption capacity of rat osteoclasts cultured by two methods [J]. Chinese Journal of Tissue Engineering Research, 2013, 17(20): 3611-3617.

Figures/Tables 6

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